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[DOI] [PMC free article] [PubMed] [Google Scholar] [102].Blair SA, Kane SV, Clayburgh DR, Turner JR

This difference influences dosing frequency in published animal protocols

For topical use: trial data shows measurable skin parameter changes (elasticity, fine lines, hydration) at 8 to 16 weeks of consistent use

Most practitioners recommend starting at 250-300 mcg daily to assess tolerance, then potentially increasing to 400-500 mcg if initial response is suboptimal and no side effects occur